The section headings (Abstract, Introduction, etc.) should be centered and the body of each section should follow immediately below the heading. Do not begin each section on a new page. If one section ends part of the way down the page, the next section heading follows immediately on the same page.
One important general rule to keep in mind is that a scientific paper is a report about something that has been done in the past. Most of the paper should be written in the PAST TENSE (was, were). The present tense (is, are) is used when stating generalizations or conclusions. The present tense is most often used in the Introduction, Discussion and Conclusion sections of papers. The paper should read as a narrative in which the author describes what was done and what results were obtained from that work.
Every scientific paper must have a self-explanatory title. By reading the title, the work being reported should be clear to the reader without having to read the paper itself. The title, "A Biology Lab Report", tells the reader nothing. An example of a good, self-explanatory title would be: "The Effects of Light and Temperature on the Growth of Populations of the Bacterium, Escherichia coli ". This title reports exactly what the researcher has done by stating three things:
If the title had been only "Effects of Light and Temperature on Escherichia coli ", the reader would have to guess which parameters were measured. (That is, were the effects on reproduction, survival, dry weight or something else?) If the title had been "Effect of Environmental Factors on Growth of Escherichia coli ", the reader would not know which environmental factors were manipulated. If the title had been "Effects of Light and Temperature on the Growth of an Organism", then the reader would not know which organism was studied. In any of the above cases, the reader would be forced to read more of the paper to understand what the researcher had done.
Exceptions do occur: If several factors were manipulated, all of them do not have to be listed. Instead, "Effects of Several Environmental Factors on Growth of Populations ofEscherichia coli " (if more than two or three factors were manipulated) would be appropriate. The same applies if more than two or three organisms were studied. For example, "Effects of Light and Temperature on the Growth of Four Species of Bacteria" would be correct. The researcher would then include the names of the bacteria in the Materials and Methods section of the paper.
The abstract section in a scientific paper is a concise digest of the content of the paper. An abstract is more than a summary. A summary is a brief restatement of preceding text that is intended to orient a reader who has studied the preceding text. An abstract is intended to be self-explanatory without reference to the paper, but is not a substitute for the paper.
The abstract should present, in about 250 words, the purpose of the paper, general materials and methods (including, if any, the scientific and common names of organisms), summarized results, and the major conclusions. Do not include any information that is not contained in the body of the paper. Exclude detailed descriptions of organisms, materials and methods. Tables or figures, references to tables or figures, or references to literature cited usually are not included in this section. The abstract is usually written last. An easy way to write the abstract is to extract the most important points from each section of the paper and then use those points to construct a brief description of your study.
The Introduction is the statement of the problem that you investigated. It should give readers enough information to appreciate your specific objectives within a larger theoretical framework. After placing your work in a broader context, you should state the specific question(s) to be answered. This section may also include background information about the problem such as a summary of any research that has been done on the problem in the past and how the present experiment will help to clarify or expand the knowledge in this general area. All background information gathered from other sources must, of course, be appropriately cited. (Proper citation of references will be described later.)
A helpful strategy in this section is to go from the general, theoretical framework to your specific question. However, do not make the Introduction too broad. Remember that you are writing for classmates who have knowledge similar to yours. Present only the most relevant ideas and get quickly to the point of the paper. For examples, see the Appendix.
MATERIALS AND METHODS
This section explains how and, where relevant, when the experiment was done. The researcher describes the experimental design, the apparatus, methods of gathering data and type of control. If any work was done in a natural habitat, the worker describes the study area, states its location and explains when the work was done. If specimens were collected for study, where and when that material was collected are stated. The general rule to remember is that the Materials and Methods section should be detailed and clear enough so that any reader knowledgeable in basic scientific techniques could duplicate the study if she/he wished to do so. For examples, see the Appendix.
DO NOT write this section as though it were directions in a laboratory exercise book. Instead of writing:
Simply describe how the experiment was done:
Also, DO NOT LIST the equipment used in the experiment. The materials that were used in the research are simply mentioned in the narrative as the experimental procedure is described in detail. If well-known methods were used without changes, simply name the methods (e.g., standard microscopic techniques; standard spectrophotometric techniques). If modified standard techniques were used, describe the changes.
Here the researcher presents summarized data for inspection using narrative text and, where appropriate, tables and figures to display summarized data. Only the results are presented. No interpretation of the data or conclusions about what the data might mean are given in this section. Data assembled in tables and/or figures should supplement the text and present the data in an easily understandable form. Do not present raw data! If tables and/or figures are used, they must be accompanied by narrative text. Do not repeat extensively in the text the data you have presented in tables and figures. But, do not restrict yourself to passing comments either. (For example, only stating that "Results are shown in Table 1." is not appropriate.) The text describes the data presented in the tables and figures and calls attention to the important data that the researcher will discuss in the Discussion section and will use to support Conclusions. (Rules to follow when constructing and presenting figures and tables are presented in a later section of this guide.)
Here, the researcher interprets the data in terms of any patterns that were observed, any relationships among experimental variables that are important and any correlations between variables that are discernible. The author should include any explanations of how the results differed from those hypothesized, or how the results were either different from or similar to those of any related experiments performed by other researchers. Remember that experiments do not always need to show major differences or trends to be important. "Negative" results also need to be explained and may represent something important--perhaps a new or changed focus for your research.
A useful strategy in discussing your experiment is to relate your specific results back to the broad theoretical context presented in the Introduction. Since your Introduction went from the general to a specific question, going from the specific back to the general will help to tie your ideas and arguments together.
This section simply states what the researcher thinks the data mean, and, as such, should relate directly back to the problem/question stated in the introduction. This section should not offer any reasons for those particular conclusions--these should have been presented in the Discussion section. By looking at only the Introduction and Conclusions sections, a reader should have a good idea of what the researcher has investigated and discovered even though the specific details of how the work was done would not be known.
In this section you should give credit to people who have helped you with the research or with writing the paper. If your work has been supported by a grant, you would also give credit for that in this section.
This section lists, in alphabetical order by author, all published information that was referred to anywhere in the text of the paper. It provides the readers with the information needed should they want to refer to the original literature on the general problem. Note that the Literature Cited section includes only those references that were actually mentioned (cited) in the paper. Any other information that the researcher may have read about the problem but did not mention in the paper is not included in this section. This is why the section is called "Literature Cited" instead of "References" or "Bibliography".
The system of citing reference material in scientific journals varies with the particular journal. The method that you will follow is the "author-date" system. Listed below are several examples of how citations should be presented in the text of your paper. The name(s) of the author(s) and year of publication are included in the body of the text. Sentence structure determines the placement of the parentheses.
WRITING A SCIENTIFIC RESEARCH ARTICLE
| Format for the paper | Edit your paper! | Useful books |FORMAT FOR THE PAPER
Scientific research articles provide a method for scientists to communicate with other scientists about the results of their research. A standard format is used for these articles, in which the author presents the research in an orderly, logical manner. This doesn't necessarily reflect the order in which you did or thought about the work. This format is:
| Title | Authors | Introduction | Materials and Methods | Results (with Tables and Figures) | Discussion | Acknowledgments | Literature Cited |
- Make your title specific enough to describe the contents of the paper, but not so technical that only specialists will understand. The title should be appropriate for the intended audience.
- The title usually describes the subject matter of the article: Effect of Smoking on Academic Performance"
- Sometimes a title that summarizes the results is more effective: Students Who Smoke Get Lower Grades"
1. The person who did the work and wrote the paper is generally listed as the first author of a research paper.
2. For published articles, other people who made substantial contributions to the work are also listed as authors. Ask your mentor's permission before including his/her name as co-author.ABSTRACT
1. An abstract, or summary, is published together with a research article, giving the reader a "preview" of what's to come. Such abstracts may also be published separately in bibliographical sources, such as Biologic al Abstracts. They allow other scientists to quickly scan the large scientific literature, and decide which articles they want to read in depth. The abstract should be a little less technical than the article itself; you don't want to dissuade your potent ial audience from reading your paper.
2. Your abstract should be one paragraph, of 100-250 words, which summarizes the purpose, methods, results and conclusions of the paper.
3. It is not easy to include all this information in just a few words. Start by writing a summary that includes whatever you think is important, and then gradually prune it down to size by removing unnecessary words, while still retaini ng the necessary concepts.
3. Don't use abbreviations or citations in the abstract. It should be able to stand alone without any footnotes.INTRODUCTION
What question did you ask in your experiment? Why is it interesting? The introduction summarizes the relevant literature so that the reader will understand why you were interested in the question you asked. One to fo ur paragraphs should be enough. End with a sentence explaining the specific question you asked in this experiment.MATERIALS AND METHODS
1. How did you answer this question? There should be enough information here to allow another scientist to repeat your experiment. Look at other papers that have been published in your field to get some idea of what is included in this section.
2. If you had a complicated protocol, it may helpful to include a diagram, table or flowchart to explain the methods you used.
3. Do not put results in this section. You may, however, include preliminary results that were used to design the main experiment that you are reporting on. ("In a preliminary study, I observed the owls for one week, and found that 73 % of their locomotor activity occurred during the night, and so I conducted all subsequent experiments between 11 pm and 6 am.")
4. Mention relevant ethical considerations. If you used human subjects, did they consent to participate. If you used animals, what measures did you take to minimize pain?RESULTS
1. This is where you present the results you've gotten. Use graphs and tables if appropriate, but also summarize your main findings in the text. Do NOT discuss the results or speculate as to why something happened; t hat goes in th e Discussion.
2. You don't necessarily have to include all the data you've gotten during the semester. This isn't a diary.
3. Use appropriate methods of showing data. Don't try to manipulate the data to make it look like you did more than you actually did.
"The drug cured 1/3 of the infected mice, another 1/3 were not affected, and the third mouse got away."TABLES AND GRAPHS
1. If you present your data in a table or graph, include a title describing what's in the table ("Enzyme activity at various temperatures", not "My results".) For graphs, you should also label the x and y axes.
2. Don't use a table or graph just to be "fancy". If you can summarize the information in one sentence, then a table or graph is not necessary.DISCUSSION
1. Highlight the most significant results, but don't just repeat what you've written in the Results section. How do these results relate to the original question? Do the data support your hypothesis? Are your results consistent with what other investigators have reported? If your results were unexpected, try to explain why. Is there another way to interpret your results? What further research would be necessary to answer the questions raised by your results? How do y our results fit into the big picture?
2. End with a one-sentence summary of your conclusion, emphasizing why it is relevant.ACKNOWLEDGMENTS
This section is optional. You can thank those who either helped with the experiments, or made other important contributions, such as discussing the protocol, commenting on the manuscript, or buying you pizza.REFERENCES (LITERATURE CITED)
There are several possible ways to organize this section. Here is one commonly used way:
1. In the text, cite the literature in the appropriate places:
Scarlet (1990) thought that the gene was present only in yeast, but it has since been identified in the platypus (Indigo and Mauve, 1994) and wombat (Magenta, et al., 1995).
2. In the References section list citations in alphabetical order.
Indigo, A. C., and Mauve, B. E. 1994. Queer place for qwerty: gene isolation from the platypus. Science 275, 1213-1214.
Magenta, S. T., Sepia, X., and Turquoise, U. 1995. Wombat genetics. In: Widiculous Wombats, Violet, Q., ed. New York: Columbia University Press. p 123-145.
Scarlet, S.L. 1990. Isolation of qwerty gene from S. cerevisae. Journal of Unusual Results 36, 26-31.
EDIT YOUR PAPER!!!
"In my writing, I average about ten pages a day. Unfortunately, they're all the same page."
A major part of any writing assignment consists of re-writing.
- Scientific writing must be accurate. Although writing instructors may tell you not to use the same word twice in a sentence, it's okay for scientific writing, which must be accurate. (A student who tried not to repeat the word "hamster" produced this confusing sentence: "When I put the hamster in a cage with the other animals, the little mammals began to play.")
- Make sure you say what you mean.
- Be careful with commonly confused words:
Instead of: The rats were injected with the drug. (sounds like a syringe was filled with drug and ground-up rats and both were injected together)
Write: I injected the drug into the rat.
Temperature has an effect on the reaction.
Temperature affects the reaction.
I used solutions in various concentrations. (The solutions were 5 mg/ml, 10 mg/ml, and 15 mg/ml)
I used solutions in varying concentrations. (The concentrations I used changed; sometimes they were 5 mg/ml, other times they were 15 mg/ml.)
Less food (can't count numbers of food)
Fewer animals (can count numbers of animals)
A large amount of food (can't count them)
A large number of animals (can count them)
The erythrocytes, which are in the blood, contain hemoglobin.
The erythrocytes that are in the blood contain hemoglobin. (Wrong. This sentence implies that there are erythrocytes elsewhere that don't contain hemoglobin.)
1. Write at a level that's appropriate for your audience.
"Like a pigeon, something to admire as long as it isn't over your head." Anonymous
2. Use the active voice. It's clearer and more concise than the passive voice.
Instead of: An increased appetite was manifested by the rats and an increase in body weight was measured.
Write: The rats ate more and gained weight.
3. Use the first person.
Instead of: It is thought
Write: I think
Instead of: The samples were analyzed
Write: I analyzed the samples
4. Avoid dangling participles.
"After incubating at 30 degrees C, we examined the petri plates." (You must've been pretty warm in there.)
1. Use verbs instead of abstract nouns
Instead of: take into consideration
2. Use strong verbs instead of "to be"
Instead of: The enzyme was found to be the active agent in catalyzing...
Write: The enzyme catalyzed...
3. Use short words.
"I would never use a long word where a short one would answer the purpose. I know there are professors in this country who 'ligate' arteries. Other surgeons tie them, and it stops the bleeding just as well."
have sufficient enough utilize use demonstrate show assistance help terminate end
4. Use concise terms.
Instead of: Write: prior to before due to the fact that because in a considerable number of cases often the vast majority of most during the time that when in close proximity to near it has long been known that I'm too lazy to look up the reference
5. Use short sentences. A sentence made of more than 40 words should probably be rewritten as two sentences.
"The conjunction 'and' commonly serves to indicate that the writer's mind still functions even when no signs of the phenomenon are noticeable." Rudolf Virchow, 1928
Check your grammar, spelling and punctuation
1. Use a spellchecker, but be aware that they don't catch all mistakes.
"When we consider the animal as a hole,..." Student's paper
2. Your spellchecker may not recognize scientific terms. For the correct spelling, try Biotech's Life Science Dictionary or one of the technical dictionaries on the reference shelf in the Biology or Health Sciences libraries.
3. Don't, use, unnecessary, commas.
4. Proofread carefully to see if you any words out.
Victoria E. McMillan, Writing Papers in the Biological Sciences, Bedford Books, Boston, 1997
The best. On sale for about $18 at Labyrinth Books, 112th Street. On reserve in Biology Library
Jan A. Pechenik, A Short Guide to Writing About Biology, Boston: Little, Brown, 1987
Harrison W. Ambrose, III & Katharine Peckham Ambrose, A Handbook of Biological Investigation, 4th edition, Hunter Textbooks Inc, Winston-Salem, 1987
Particularly useful if you need to use statistics to analyze your data. Copy on Reference shelf in Biology Library.
Robert S. Day, How to Write and Publish a Scientific Paper, 4th edition, Oryx Press, Phoenix, 1994.
Earlier editions also good. A bit more advanced, intended for those writing papers for publication. Fun to read. Several copies available in Columbia libraries.
William Strunk, Jr. and E. B. White, The Elements of Style, 3rd ed. Macmillan, New York, 1987.
Several copies available in Columbia libraries. Strunk's first edition is available on-line.